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返回到 冷冻电子显微镜(cryo-EM)入门

學生對 加州理工学院 提供的 冷冻电子显微镜(cryo-EM)入门 的評價和反饋

4.9
245 個評分
44 條評論

課程概述

This class covers the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. Philosophy: The course emphasizes concepts rather than mathematical details, taught through numerous drawings and example images. It is meant for anyone interested in the burgeoning fields of cryo-EM and 3-D EM, including cell biologists or molecular biologists without extensive training in mathematics or imaging physics and practicing electron microscopists who want to broaden their understanding of the field. The class is perfect as a primer for anyone who is about to be trained as a cryo-electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo-EM meetings. Pre-requisites: The recommended prerequisites are college-freshman-level math, physics, and biochemistry. Pace: There are 14.5 hours of lecture videos total separated into 40 individual “modules” lasting on average 20 minutes each. Each module has at the end a list of “concept check” questions you can use to test your knowledge of what was presented. As the modules are grouped into seven major subjects, one reasonable plan would be to go through one major subject each day. That would mean watching a couple hours of lecture and spending another hour or so thinking through the concept check questions each day for a week. Another reasonable plan would be to go through one module each day for a little over a month, or even three modules a week (Monday, Wednesday, and Friday) for a 3-month term. It is likely that as you then move on to actually begin using a cryo-EM or otherwise engage in the field, you will want to repeat certain modules....

熱門審閱

LR

Feb 19, 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.

AM

Jul 20, 2016

An excellent introduction to the topic, explaining from the basics to the detail of things. Useful and recommended to anyone in structural biology.

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26 - 冷冻电子显微镜(cryo-EM)入门 的 44 個評論(共 44 個)

創建者 Nivetha R

Oct 12, 2019

Extremely Helpful and very comfortable to learn

創建者 Xiaolong C

Dec 23, 2015

very great course, including so much details.

創建者 Bing W

May 18, 2016

It's really useful. Thanks so much.

創建者 赵炜

Oct 25, 2015

The best EM course available online

創建者 ANIL K S

Sep 12, 2018

very good for the beginner like me

創建者 Baharak

Dec 27, 2015

It" Awesome. Thank you.

創建者 Chao-Han Y

Oct 28, 2015

USEFUL THANK CALTECH :)

創建者 Francois T

Aug 17, 2016

Thank you Dr. Jensen!

創建者 董炜哲

Sep 04, 2015

absolutely fantastic!

創建者 jadson c d s

Dec 28, 2015

Simply AMAZING!

創建者 Shokir A P

Sep 13, 2015

great course!

創建者 stanwang

Oct 19, 2017

excellent

創建者 Scott C

Nov 29, 2015

Very good

創建者 minagquan

Sep 10, 2015

good

創建者 Andrew L P

Aug 19, 2015

Just needs a certificate and some practice concept questsions added and would be perfect. Right now just really interesting videos for the intermediate mind...not much math...mostly concepts so good for the beginner who wants to know more. :)

創建者 Herman A

Feb 01, 2016

Great information, great teacher, greater visual presentation. Disclaimer: I only took the first week.

創建者 Marcus G

Jan 02, 2016

Great introduction to Cryo-EM for biologists that covers all the basics in a clear and concise manner.

創建者 Haris F

Jan 11, 2016

Pioneering online course in Electron Microscopy.

創建者 Christine L

Oct 19, 2016

Excellent content