課程信息
4.8
18 個評分
6 個審閱

100% 在線

立即開始,按照自己的計劃學習。

可靈活調整截止日期

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中級

None required but you should be familiar with basic aspects of plant and molecular biology. Bioinformatic Methods I and II would be good preparation

完成時間大約為11 小時

建議:6 weeks of hands-on study, plus 2 evaluation weeks. The hands-on modules will take 2-4 hours of time...

英語(English)

字幕:英語(English)

100% 在線

立即開始,按照自己的計劃學習。

可靈活調整截止日期

根據您的日程表重置截止日期。

中級

None required but you should be familiar with basic aspects of plant and molecular biology. Bioinformatic Methods I and II would be good preparation

完成時間大約為11 小時

建議:6 weeks of hands-on study, plus 2 evaluation weeks. The hands-on modules will take 2-4 hours of time...

英語(English)

字幕:英語(English)

教學大綱 - 您將從這門課程中學到什麼

1
完成時間為 3 小時

Plant Genomic Databases, and useful sites for info about proteins

In this module we'll be exploring several plant databases including Ensembl Plants, Gramene, PLAZA, SUBA, TAIR and Araport. The information in these databases allows us to easily identify functional regions within gene products, view subcellular localization, find homologs in other species, and even explore pre-computed gene trees to see if our gene of interest has undergone a gene duplication event in another species, all at the click of a mouse!...
4 個視頻 (總計 44 分鐘), 4 個閱讀材料, 1 個測驗
4 個視頻
Mini-lecture17分鐘
Lab Discussion23分鐘
Summary1分鐘
4 個閱讀材料
Acknowledgements10分鐘
Course Logistics10分鐘
Mini-lecture Notes10分鐘
Lab 1 -- Plant Genomic Databases and Tools for Exploring Protein Information30分鐘
1 個練習
Lab Quiz 1
2
完成時間為 2 小時

Expression Analysis

Vast databases of gene expression and nifty visualization tools allow us to explore where and when a gene is expressed. Often this information can be used to help guide a search for a phenotype if we don't see a phenotype in a gene mutant under "normal" growth conditions. We explore several tools for Arabidopsis data (eFP Browser, Genevestigator, TraVA DB, Araport) along with NCBI's Genome Data Viewer for RNA-seq data for other plant species. We also examine the MPSS database of small RNAs and degradation products to see if our example gene has any potential microRNA targets....
4 個視頻 (總計 38 分鐘), 2 個閱讀材料, 1 個測驗
4 個視頻
Mini-lecture17分鐘
Lab Discussion18分鐘
Summary50
2 個閱讀材料
Mini-lecture Notes10分鐘
Lab 2 -- Expression Analysis30分鐘
1 個練習
Lab Quiz 2
3
完成時間為 2 小時

Coexpression Tools

Being able to group genes by similar patterns of expression across expression data sets using algorithms like WGCNA is a very useful way of organizing the data. Clusters of genes with similar patterns of expression can then be subject to Gene Ontology term enrichment analysis (see Module 5) or examined to see if they are part of the same pathway. What's even more powerful is being able to identify genes with similar patterns of expression without doing a single expression profiling experiment, by mining gene expression databases! There are several tools that allow you to do this in many plant species simply by entering a query gene identifier. The genes that are returned are often in the same biological process as the query gene, and thus this "guilt-by-association" paradigm is a excellent tool for hypothesis generation....
4 個視頻 (總計 29 分鐘), 2 個閱讀材料, 1 個測驗
4 個視頻
Mini-lecture9分鐘
Lab Discussion16分鐘
Summary49
2 個閱讀材料
Mini-lecture Notes10分鐘
Lab 3 -- Coexpression Tools30分鐘
1 個練習
Lab Quiz 3
4

Sectional Quiz 1

...
1 個測驗
1 個練習
Sectional Quiz 1
4.8
6 個審閱Chevron Right

熱門審閱

創建者 QRMar 10th 2019

Very good course for mastering bioinformatics skills. Genetics and molecular biology students/professionals should take this course to augment their research skills.

創建者 FSFeb 22nd 2019

Professor Provart is very good and the labs taught us in simple and direct instructions how to perform a wide range of analysis regarding Plant Bioinformatics.

講師

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Nicholas James Provart

Professor
Cell & Systems Biology

關於 多伦多大学

Established in 1827, the University of Toronto is one of the world’s leading universities, renowned for its excellence in teaching, research, innovation and entrepreneurship, as well as its impact on economic prosperity and social well-being around the globe. ...

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