課程信息
4.7
121 個評分
30 個審閱
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100% 在線

立即開始,按照自己的計劃學習。
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可靈活調整截止日期

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中級

中級

完成時間(小時)

完成時間大約為13 小時

建議:5 weeks, 2-3 hours/week...
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英語(English)

字幕:英語(English)

您將獲得的技能

BiologySample PreparationSample CollectionCell Biology
100% 在線

100% 在線

立即開始,按照自己的計劃學習。
可靈活調整截止日期

可靈活調整截止日期

根據您的日程表重置截止日期。
中級

中級

完成時間(小時)

完成時間大約為13 小時

建議:5 weeks, 2-3 hours/week...
可選語言

英語(English)

字幕:英語(English)

教學大綱 - 您將從這門課程中學到什麼

1
完成時間(小時)
完成時間為 2 小時

Introduction to the Course and the Field of Extracellular Vesicles

In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles. ...
Reading
4 個視頻 (總計 69 分鐘), 1 個閱讀材料, 1 個測驗
Video4 個視頻
Introduction to the Course11分鐘
The Origin of EVs throughout the Phylogenetic Tree11分鐘
History of Exosomes and Extracellular Vesicles32分鐘
Reading1 個閱讀材料
Syllabus10分鐘
Quiz1 個練習
Introduction to Extracellular Vesicles18分鐘
2
完成時間(小時)
完成時間為 3 小時

Biogenesis, Cargo and Uptake of Extracellular Vesicles

In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types present in extracellular vesicles as well as a brief overview what the functions of these molecules are. Furthermore, the techniques that are commonly used to detect these molecules as well as databases that can be useful to use when analyzing the cargo of extracellular vesicles will be highlighted....
Reading
6 個視頻 (總計 142 分鐘), 1 個測驗
Video6 個視頻
Mechanisms of Extracellular Vesicle Uptake - Part 122分鐘
Mechanisms of Extracellular Vesicle Uptake - Part 223分鐘
The Protein Content of Extracellular Vesicles23分鐘
The RNA Content of Extracellular Vesicles35分鐘
The Lipid Content of Extracellular Vesicles18分鐘
Quiz1 個練習
Biogenesis, Cargo and Uptake of Extracellular Vesicles30分鐘
3
完成時間(小時)
完成時間為 2 小時

Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles

In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring material and isolation of extracellular vesicles. This module will help you with the different choices, such as anticoagulants, collection time points and protein inhibitors, which are important for the outcome when working with a particular body fluid compared to conditional media or other body fluids. This module will also illustrate some example of studies on extracellular vesicles from body fluid such as blood, urine, breast milk and cerebrospinal fluid and why it is of interest to analyse extracellular vesicles from these body fluids. ...
Reading
5 個視頻 (總計 79 分鐘), 1 個測驗
Video5 個視頻
Blood Plasma and Serum15分鐘
Breast Milk8分鐘
Urine25分鐘
Cerebrospinal Fluid15分鐘
Quiz1 個練習
Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles36分鐘
4
完成時間(小時)
完成時間為 2 小時

Methods for Isolating Extracellular Vesicles

This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits....
Reading
6 個視頻 (總計 77 分鐘), 1 個測驗
Video6 個視頻
Differential Ultracentrifugation - Part 216分鐘
Density Gradient12分鐘
Size Exclusion Chromatography14分鐘
Kit-based Precipitation12分鐘
Summary of Isolation Methods for Extracellular Vesicles6分鐘
Quiz1 個練習
Methods for Isolating Extracellular Vesicles22分鐘

講師

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Cecilia Lasser

Researcher
Krefting Research Centre, University of Gothenburg

關於 加州大学尔湾分校

Since 1965, the University of California, Irvine has combined the strengths of a major research university with the bounty of an incomparable Southern California location. UCI’s unyielding commitment to rigorous academics, cutting-edge research, and leadership and character development makes the campus a driving force for innovation and discovery that serves our local, national and global communities in many ways....

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