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返回到 冷冻电子显微镜(cryo-EM)入门

學生對 加州理工学院 提供的 冷冻电子显微镜(cryo-EM)入门 的評價和反饋

241 個評分
43 個審閱


This class covers the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. Philosophy: The course emphasizes concepts rather than mathematical details, taught through numerous drawings and example images. It is meant for anyone interested in the burgeoning fields of cryo-EM and 3-D EM, including cell biologists or molecular biologists without extensive training in mathematics or imaging physics and practicing electron microscopists who want to broaden their understanding of the field. The class is perfect as a primer for anyone who is about to be trained as a cryo-electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo-EM meetings. Pre-requisites: The recommended prerequisites are college-freshman-level math, physics, and biochemistry. Pace: There are 14.5 hours of lecture videos total separated into 40 individual “modules” lasting on average 20 minutes each. Each module has at the end a list of “concept check” questions you can use to test your knowledge of what was presented. As the modules are grouped into seven major subjects, one reasonable plan would be to go through one major subject each day. That would mean watching a couple hours of lecture and spending another hour or so thinking through the concept check questions each day for a week. Another reasonable plan would be to go through one module each day for a little over a month, or even three modules a week (Monday, Wednesday, and Friday) for a 3-month term. It is likely that as you then move on to actually begin using a cryo-EM or otherwise engage in the field, you will want to repeat certain modules....



Feb 19, 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.


Jul 20, 2016

An excellent introduction to the topic, explaining from the basics to the detail of things. Useful and recommended to anyone in structural biology.


26 - 冷冻电子显微镜(cryo-EM)入门 的 43 個評論(共 43 個)

創建者 Nikos N

Oct 30, 2017

CryoEM made crystal clear! ;P

創建者 Bryant A

May 16, 2016

Very well detailed on a easy to learn pase. Will help you learn all to EM that is necessary before using them.

創建者 Bing W

May 18, 2016

It's really useful. Thanks so much.

創建者 Syed O A

Nov 26, 2015

best course for lab workers ,physics is deal in a great detail

創建者 Xiaolong C

Dec 23, 2015

very great course, including so much details.

創建者 Baharak

Dec 27, 2015

It" Awesome. Thank you.

創建者 Yulia R

Aug 28, 2015

Very clear, very easy to understand, a lot of useful information. Excellent course.

創建者 sreenu b

Aug 11, 2016

Excellent class and Presentation with all clarity concepts

創建者 Chao-Han Y

Oct 28, 2015


創建者 Kuntamukkala P K

Aug 14, 2015

I highly recommend this course to my Post Graduating Friends and Research Scholars. This course helped me so much in understanding EM operation which is not available any where else. It is helping me in current Thesis work. Professor of this Course is explaining in a lucid manner. Thank you very much Professor Grant Jensen. Thank you Coursera.

創建者 Robert F

Apr 04, 2017

Great course with clear explanations and very entertaining topics! Didn't expect to cover so many aspects of cryo-EM, would recommend it to everyone interested/working in structural biology or science in general.

創建者 William N

Aug 31, 2018

Loved it. Although it's not heavy on math it helps to have a basic understanding of waves, how they propagate, how they are mathematically represented by sin/cos functions and how interference between two waves works.

創建者 Nivetha R

Oct 12, 2019

Extremely Helpful and very comfortable to learn

創建者 Herman A

Feb 01, 2016

Great information, great teacher, greater visual presentation. Disclaimer: I only took the first week.

創建者 Marcus G

Jan 02, 2016

Great introduction to Cryo-EM for biologists that covers all the basics in a clear and concise manner.

創建者 Andrew L P

Aug 19, 2015

Just needs a certificate and some practice concept questsions added and would be perfect. Right now just really interesting videos for the intermediate mind...not much math...mostly concepts so good for the beginner who wants to know more. :)

創建者 Christine L

Oct 19, 2016

Excellent content

創建者 Haris F

Jan 11, 2016

Pioneering online course in Electron Microscopy.