Bioinformatic Methods II

提供方

Bioinformatic Methods II

University of Toronto

Large-scale biology projects such as the sequencing of the human genome and gene expression surveys using RNA-seq, microarrays and other technologies have created a wealth of data for biologists. However, the challenge facing scientists is analyzing and even accessing these data to extract useful information pertaining to the system being studied. This course focuses on employing existing bioinformatic resources – mainly web-based programs and databases – to access the wealth of data to answer questions relevant to the average biologist, and is highly hands-on. Topics covered include multiple sequence alignments, phylogenetics, gene expression data analysis, and protein interaction networks, in two separate parts. The first part, Bioinformatic Methods I, dealt with databases, Blast, multiple sequence alignments, phylogenetics, selection analysis and metagenomics. This, the second part, Bioinformatic Methods II, will cover motif searching, protein-protein interactions, structural bioinformatics, gene expression data analysis, and cis-element predictions. This pair of courses is useful to any student considering graduate school in the biological sciences, as well as students considering molecular medicine. These courses are based on one taught at the University of Toronto to upper-level undergraduates who have some understanding of basic molecular biology. If you're not familiar with this, something like https://learn.saylor.org/course/bio101 might be helpful. No programming is required for this course although some command line work (though within a web browser) occurs in the 5th module....

100% 在線課程

立即開始，按照自己的計劃學習。

可靈活調整截止日期

根據您的日程表重置截止日期。

建議：12-18 hours of videos and labs

完成時間大約為11 小時

English

字幕：English

100% 在線課程

立即開始，按照自己的計劃學習。

可靈活調整截止日期

根據您的日程表重置截止日期。

建議：12-18 hours of videos and labs

完成時間大約為11 小時

English

字幕：English

教學大綱 - 您將從這門課程中學到什麼

1

章節

完成時間為 3 小時

Protein Motifs

In this module we'll be exploring conserved regions within protein families. Such regions can help us understand the biology of a sequence, in that they are likely important for biological function, and also be used to help ascribe function to sequences where we can't identify any homologs in the databases. There are various ways of describing the conserved regions from simple regular expressions to profiles to profile hidden Markov models (HMMs)....

4 個視頻（共 34 分鐘）, 4 個閱讀材料, 1 個測驗

4 個視頻

Introduction1分鐘

Lecture21分鐘

Lab Discussion9分鐘

Summary1分鐘

4 個閱讀材料

Acknowledgements10分鐘

Course Logistics10分鐘

Lecture Materials10分鐘

Lab 1 -- Protein Domain, Motif and Profile Analysis30分鐘

1 個練習

Lab 1 Quiz6分鐘

2

章節

完成時間為 2 小時

Protein-Protein Interactions

In this module we'll be exploring protein-protein interactions (PPIs). Protein-protein interactions are important as proteins don't act in isolation, and often an examination of the interaction partners (determined in an unbiased, perhaps high throughput way) of a given protein can tell us a lot about its biology. We'll talk about some different methods used to determine PPIs and go over their strengths and weaknesses. In the lab we'll use 3 different tools and two different databases to examine interaction partners of BRCA2, a protein that we examined in last module's lab. Finally, we'll touch on a "foundational" concept, Gene Ontology (GO) term enrichment analysis, to help us understand in an overview way the proteins interacting with our example....

4 個視頻（共 37 分鐘）, 2 個閱讀材料, 1 個測驗

4 個視頻

Introduction1分鐘

Lecture23分鐘

Lab Discussion11分鐘

Summary1分鐘

2 個閱讀材料

Lecture Materials10分鐘

Lab 2 -- Protein-Protein Interactions30分鐘

1 個練習

Lab 2 Quiz6分鐘

3

章節

完成時間為 2 小時

Protein Structure

The determination of a protein's tertiary structure in three dimensions can tell us a lot about the biology of that protein. In this module's mini-lecture, we'll talk about some different methods used to determine a protein's tertiary structure and cover the main database for protein structure data, the PDB. In the lab we'll explore the PDB and an online tool for searching for structural (as opposed to sequence) similarity, VAST. We'll then use a nice piece of stand-alone software, PyMOL, to explore several protein structures in more detail....

4 個視頻（共 32 分鐘）, 2 個閱讀材料, 1 個測驗

4 個視頻

Introduction1分鐘

Lecture13分鐘

Lab Discussion16分鐘

Summary1分鐘

2 個閱讀材料

Lecture Materials10分鐘

Lab 3 -- Structural Bioinformatics30分鐘

1 個練習

Lab 3 Quiz6分鐘

4

章節

完成時間為 10 分鐘

Review: Protein Motifs, Protein-Protein Interactions, and Protein Structure

...

1 個測驗

1 個練習

Quiz: Protein Motifs, Protein-Protein Interactions, and Protein Structure10分鐘

講師

Nicholas James Provart

Professor

Cell & Systems Biology

關於 University of Toronto

Established in 1827, the University of Toronto has one of the strongest research and teaching faculties in North America, presenting top students at all levels with an intellectual environment unmatched in depth and breadth on any other Canadian campus. ...

常見問題

When will I have access to the lectures and assignments?
Once you enroll for a Certificate, you’ll have access to all videos, quizzes, and programming assignments (if applicable). Peer review assignments can only be submitted and reviewed once your session has begun. If you choose to explore the course without purchasing, you may not be able to access certain assignments.
What will I get if I purchase the Certificate?
When you purchase a Certificate you get access to all course materials, including graded assignments. Upon completing the course, your electronic Certificate will be added to your Accomplishments page - from there, you can print your Certificate or add it to your LinkedIn profile. If you only want to read and view the course content, you can audit the course for free.
What is the refund policy?
Is financial aid available?

還有其他問題嗎？請訪問學生幫助中心。

Bioinformatic Methods II

Bioinformatic Methods II

100% 在線課程

可靈活調整截止日期

建議：12-18 hours of videos and labs

English

100% 在線課程

可靈活調整截止日期

建議：12-18 hours of videos and labs

English

教學大綱 - 您將從這門課程中學到什麼

1

Protein Motifs

2

Protein-Protein Interactions

3

Protein Structure

4

Review: Protein Motifs, Protein-Protein Interactions, and Protein Structure

25%

83%

12%

熱門審閱

講師

Nicholas James Provart

關於 University of Toronto

常見問題