關於此課程：Large-scale biology projects such as the sequencing of the human genome and gene expression surveys using RNA-seq, microarrays and other technologies have created a wealth of data for biologists. However, the challenge facing scientists is analyzing and even accessing these data to extract useful information pertaining to the system being studied. This course focuses on employing existing bioinformatic resources – mainly web-based programs and databases – to access the wealth of data to answer questions relevant to the average biologist, and is highly hands-on. Topics covered include multiple sequence alignments, phylogenetics, gene expression data analysis, and protein interaction networks, in two separate parts. The first part, Bioinformatic Methods I, dealt with databases, Blast, multiple sequence alignments, phylogenetics, selection analysis and metagenomics. This, the second part, Bioinformatic Methods II, will cover motif searching, protein-protein interactions, structural bioinformatics, gene expression data analysis, and cis-element predictions. This pair of courses is useful to any student considering graduate school in the biological sciences, as well as students considering molecular medicine. These courses are based on one taught at the University of Toronto to upper-level undergraduates who have some understanding of basic molecular biology. If you're not familiar with this, something like https://learn.saylor.org/course/bio101 might be helpful. No programming is required for this course although some command line work (though within a web browser) occurs in the 5th module.
製作方： University of Toronto
教學方： Nicholas James Provart, Professor
Cell & Systems Biology
| 承諾學習時間 | 12-18 hours of videos and labs |
| 語言 | English |
| 如何通過 | 通過所有計分作業以完成課程。 |
| 用戶評分 |
授課大綱
第 1 週
Protein Motifs
In this module we'll be exploring conserved regions within protein families. Such regions can help us understand the biology of a sequence, in that they are likely important for biological function, and also be used to help ascribe function to sequences where we can't identify any homologs in the databases. There are various ways of describing the conserved regions from simple regular expressions to profiles to profile hidden Markov models (HMMs).
4 個視頻, 4 個閱讀材料
- Leyendo: Acknowledgements
- Leyendo: Course Logistics
- 視頻: Lecture
- Leyendo: Lecture Materials
- Leyendo: Lab 1 -- Protein Domain, Motif and Profile Analysis
- 視頻: Lab Discussion
- 視頻: Summary
已評分: Lab 1 Quiz
第 2 週
Protein-Protein Interactions
In this module we'll be exploring protein-protein interactions (PPIs). Protein-protein interactions are important as proteins don't act in isolation, and often an examination of the interaction partners (determined in an unbiased, perhaps high throughput way) of a given protein can tell us a lot about its biology. We'll talk about some different methods used to determine PPIs and go over their strengths and weaknesses. In the lab we'll use 3 different tools and two different databases to examine interaction partners of BRCA2, a protein that we examined in last module's lab. Finally, we'll touch on a "foundational" concept, Gene Ontology (GO) term enrichment analysis, to help us understand in an overview way the proteins interacting with our example.
4 個視頻, 2 個閱讀材料
- 視頻: Lecture
- Leyendo: Lecture Materials
- Leyendo: Lab 2 -- Protein-Protein Interactions
- 視頻: Lab Discussion
- 視頻: Summary
已評分: Lab 2 Quiz
第 3 週
Protein Structure
The determination of a protein's tertiary structure in three dimensions can tell us a lot about the biology of that protein. In this module's mini-lecture, we'll talk about some different methods used to determine a protein's tertiary structure and cover the main database for protein structure data, the PDB. In the lab we'll explore the PDB and an online tool for searching for structural (as opposed to sequence) similarity, VAST. We'll then use a nice piece of stand-alone software, PyMOL, to explore several protein structures in more detail.
4 個視頻, 2 個閱讀材料
- 視頻: Lecture
- Leyendo: Lecture Materials
- Leyendo: Lab 3 -- Structural Bioinformatics
- 視頻: Lab Discussion
- 視頻: Summary
已評分: Lab 3 Quiz
第 4 週
Review: Protein Motifs, Protein-Protein Interactions, and Protein Structure
已評分: Quiz: Protein Motifs, Protein-Protein Interactions, and Protein Structure
第 5 週
Gene Expression Analysis I
When and where genes are expressed (active) in tissues or cells is one of the main determinants of what makes that tissue or cell the way it is, both in terms of morphology and in terms of response to external stimuli. Several different methods exist for generating gene expression levels for all of the genes in the genome in tissues or even at cell-type-specific resolution. In this class we'll be processing and then examining some gene expression data generated using RNA-seq. We'll explore one of the main databases for RNA-seq expression data, the Sequence Read Archive (SRA), and then use an open-source suite of programs in R called BioConductor to process the raw reads from 4 RNA-seq data sets, to summarize their expression levels, to select significantly differentially expressed genes, and finally to visualize these as a heat map.
4 個視頻, 2 個閱讀材料
- 視頻: Lecture
- Leyendo: Lecture Materials
- Leyendo: Lab 4 -- Gene Expression Analysis I
- 視頻: Lab Discusssion
- 視頻: Summary
已評分: Lab 4 Quiz
第 6 週
Gene Expression Analysis II
When and where genes are expressed (active) in tissues or cells is one of the main determinants of what makes that tissue or cell the way it is, both in terms of morphology and in terms of response to external stimuli. Several different methods exist for generating gene expression levels for all of the genes in the genome in tissues or even at cell-type-specific resolution. In this class we'll be hierarchically clustering our significantly differentially expressed genes from last time using BioConductor and the built-in function of an online tool, called Expression Browser. Then we'll be using another online tool that uses a similarity metric, the Pearson correlation coefficient, to identify genes responding in a similar manner to our gene of interest, in this case AP3. We'll use a second tool, ATTED-II to corroborate our gene list. We'll also be exploring some online databases of gene expression and an online tool for doing a Gene Ontology enrichment analysis.
5 個視頻, 2 個閱讀材料
- 視頻: Lecture part 1
- 視頻: Lecture part 2
- Leyendo: Lecture Materials
- Leyendo: Lab 5 -- Gene Expression Data Analysis II
- 視頻: Lab Discussion
- 視頻: Summary
已評分: Lab 5 Quiz
第 7 週
Cis Regulatory Systems
When and where genes are expressed in tissues or cells is one of the main determinants of what makes that tissue or cell the way it is, both in terms of morphology and in terms of response to external stimuli. Gene expression is controlled in part by the presence of short sequences in the promoters (and other parts) of genes, called cis-elements, which permit transcription factors and other regulatory proteins to bind to direct the patterns of expression in certain tissues or cells or in response to environmental stimuli: We'll explore a couple of sets of promoters of genes that are coexpressed with AP3 from Arabidopsis, and with INSULIN from human, for the presence of known cis-elements, and we'll also try to predict some new ones using a couple of different methods.
4 個視頻, 2 個閱讀材料
- 視頻: Lecture
- Leyendo: Lecture Materials
- Leyendo: Lab 6 -- Cis Regulatory Element Mapping and Prediction
- 視頻: Lab Discussion
- 視頻: Summary
已評分: Lab 6 Quiz
第 8 週
Review: Gene Expression Analysis and Cis Regulatory Systems + Final Assignment
1 個閱讀材料
- Leyendo: Final Assignment Instructions
已評分: Quiz: Modules 5-7
已評分: Final Assignment
常見問題解答
運作方式
Trabajo del curso
Cada curso es como un libro de texto interactivo, con videos pregrabados, cuestionarios y proyectos.
Ayuda de tus compañeros
Conéctate con miles de estudiantes y debate ideas y materiales del curso, y obtén ayuda para dominar los conceptos.
Certificados
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製作方
University of Toronto
Established in 1827, the University of Toronto has one of the strongest research and teaching faculties in North America, presenting top students at all levels with an intellectual environment unmatched in depth and breadth on any other Canadian campus.
評分和審閱
Hi Nicholas, Thank you so much for giving a lot of information. Bioinformatic Methods II was little difficult but understood after repeating the lad discussions. Thanks a lot.
An amazing course....highly recommended.
Truly Knowledgeable course........
I just loved the course, I learned several tools in a short time and I hope to take advantage of them in the future. This course encouraged me to continue with similar ones.
Many thanks to Professor Provart, for his simple way of explaining, I hope he continues to dictate other courses in the future.