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36 reviews
Large-scale biology projects such as the sequencing of the human genome and gene expression surveys using RNA-seq, microarrays and other technologies have created a wealth of data for biologists. However, the challenge facing scientists is analyzing and even accessing these data to extract useful information pertaining to the system being studied. This course focuses on employing existing bioinformatic resources – mainly web-based programs and databases – to access the wealth of data to answer questions relevant to the average biologist, and is highly hands-on. Topics covered include multiple sequence alignments, phylogenetics, gene expression data analysis, and protein interaction networks, in two separate parts. The first part, Bioinformatic Methods I, dealt with databases, Blast, multiple sequence alignments, phylogenetics, selection analysis and metagenomics. This, the second part, Bioinformatic Methods II, will cover motif searching, protein-protein interactions, structural bioinformatics, gene expression data analysis, and cis-element predictions. This pair of courses is useful to any student considering graduate school in the biological sciences, as well as students considering molecular medicine. These courses are based on one taught at the University of Toronto to upper-level undergraduates who have some understanding of basic molecular biology. If you're not familiar with this, something like https://learn.saylor.org/course/bio101 might be helpful. No programming is required for this course although some command line work (though within a web browser) occurs in the 5th module....
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建議:12-18 hours of videos and labs

完成時間大約為11 小時
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Globe

100% 在線課程

立即開始,按照自己的計劃學習。
Calendar

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建議:12-18 hours of videos and labs

完成時間大約為11 小時
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English

字幕:English

教學大綱 - 您將從這門課程中學到什麼

1

章節
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完成時間為 3 小時

Protein Motifs

In this module we'll be exploring conserved regions within protein families. Such regions can help us understand the biology of a sequence, in that they are likely important for biological function, and also be used to help ascribe function to sequences where we can't identify any homologs in the databases. There are various ways of describing the conserved regions from simple regular expressions to profiles to profile hidden Markov models (HMMs)....
Reading
4 個視頻(共 34 分鐘), 4 個閱讀材料, 1 個測驗
Video4 個視頻
Lecture21分鐘
Lab Discussion9分鐘
Summary1分鐘
Reading4 個閱讀材料
Acknowledgements10分鐘
Course Logistics10分鐘
Lecture Materials10分鐘
Lab 1 -- Protein Domain, Motif and Profile Analysis30分鐘
Quiz1 個練習
Lab 1 Quiz6分鐘

2

章節
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完成時間為 2 小時

Protein-Protein Interactions

In this module we'll be exploring protein-protein interactions (PPIs). Protein-protein interactions are important as proteins don't act in isolation, and often an examination of the interaction partners (determined in an unbiased, perhaps high throughput way) of a given protein can tell us a lot about its biology. We'll talk about some different methods used to determine PPIs and go over their strengths and weaknesses. In the lab we'll use 3 different tools and two different databases to examine interaction partners of BRCA2, a protein that we examined in last module's lab. Finally, we'll touch on a "foundational" concept, Gene Ontology (GO) term enrichment analysis, to help us understand in an overview way the proteins interacting with our example....
Reading
4 個視頻(共 37 分鐘), 2 個閱讀材料, 1 個測驗
Video4 個視頻
Lecture23分鐘
Lab Discussion11分鐘
Summary1分鐘
Reading2 個閱讀材料
Lecture Materials10分鐘
Lab 2 -- Protein-Protein Interactions30分鐘
Quiz1 個練習
Lab 2 Quiz6分鐘

3

章節
Clock
完成時間為 2 小時

Protein Structure

The determination of a protein's tertiary structure in three dimensions can tell us a lot about the biology of that protein. In this module's mini-lecture, we'll talk about some different methods used to determine a protein's tertiary structure and cover the main database for protein structure data, the PDB. In the lab we'll explore the PDB and an online tool for searching for structural (as opposed to sequence) similarity, VAST. We'll then use a nice piece of stand-alone software, PyMOL, to explore several protein structures in more detail....
Reading
4 個視頻(共 32 分鐘), 2 個閱讀材料, 1 個測驗
Video4 個視頻
Lecture13分鐘
Lab Discussion16分鐘
Summary1分鐘
Reading2 個閱讀材料
Lecture Materials10分鐘
Lab 3 -- Structural Bioinformatics30分鐘
Quiz1 個練習
Lab 3 Quiz6分鐘

4

章節
Clock
完成時間為 10 分鐘

Review: Protein Motifs, Protein-Protein Interactions, and Protein Structure

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1 個測驗
Quiz1 個練習
Quiz: Protein Motifs, Protein-Protein Interactions, and Protein Structure10分鐘
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創建者 RGApr 6th 2017

Gives the student real world exposure to the tools to study proteins gene regulation, etc. Instructor is involved and friendly. Highly recommended for someone who is interested in contemporary

創建者 NMJan 9th 2018

Hi Nicholas, Thank you so much for giving a lot of information. Bioinformatic Methods II was little difficult but understood after repeating the lad discussions. Thanks a lot.

講師

Nicholas James Provart

Professor
Cell & Systems Biology

關於 University of Toronto

Established in 1827, the University of Toronto has one of the strongest research and teaching faculties in North America, presenting top students at all levels with an intellectual environment unmatched in depth and breadth on any other Canadian campus. ...

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