課程信息
4.7
717 個評分
158 個審閱

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初級

完成時間大約為13 小時

建議:12-18 hours videos and labs...

英語(English)

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您將獲得的技能

Genetic AnalysisBioinformatics AnalysisEvolutionComparative Genomics

100% 在線

立即開始,按照自己的計劃學習。

可靈活調整截止日期

根據您的日程表重置截止日期。

初級

完成時間大約為13 小時

建議:12-18 hours videos and labs...

英語(English)

字幕:英語(English)

教學大綱 - 您將從這門課程中學到什麼

1
完成時間為 3 小時

NCBI/Blast I

In this module we'll be exploring the amazing resources available at NCBI, the National Centre for Biotechnology Information, run by the National Library of Medicine in the USA. We'll also be doing a Blast search to find similar sequences in the enormous NR sequence database. We can use similar sequences to infer homology, which is the primary predictor of gene or protein function. ...
4 個視頻 (總計 52 分鐘), 4 個閱讀材料, 1 個測驗
4 個視頻
Lecture25分鐘
Lab Discussion24分鐘
Summary
4 個閱讀材料
Acknowledgements10分鐘
Course Logistics10分鐘
Lecture Materials10分鐘
Lab 1 -- Exploring NCBI30分鐘
1 個練習
Lab 1 Quiz6分鐘
2
完成時間為 3 小時

Blast II/Comparative Genomics

In this module we'll continue exploring the incredible resources available at NCBI, the National Centre for Biotechnology Information. We will be performing several different kinds of Blast searches: BlastP, PSI-Blast, and Translated Blast. We can use similar sequences identified by such methods to infer homology, which is the primary predictor of gene or protein function. We'll also be comparing parts of the genomes of a couple of different species, to see how similar they are....
4 個視頻 (總計 58 分鐘), 2 個閱讀材料, 1 個測驗
4 個視頻
Lecture32分鐘
Lab Discussion23分鐘
Summary
2 個閱讀材料
Lecture Materials10分鐘
Lab 2 -- Advanced Blast and Comparative Genomics30分鐘
1 個練習
Lab 2 Quiz6分鐘
3
完成時間為 2 小時

Multiple Sequence Alignments

In this module we'll be doing multiple sequence alignments with Clustal (as implemented in MEGA), DiAlign, and MAFFT. Multiple sequences alignments can tell you where in a sequence the conserved and variable regions are, which is important for understanding the biology of the sequences under investigation. It also has practical applications, such as being able to design PCR primers that will amplify sequences from a number of different species, for example....
4 個視頻 (總計 42 分鐘), 2 個閱讀材料, 1 個測驗
4 個視頻
Lecture23分鐘
Lab Discussion16分鐘
Summary
2 個閱讀材料
Lecture Materials10分鐘
Lab 3 -- Multiple Sequence Alignment30分鐘
1 個練習
Lab 3 Quiz6分鐘
4
完成時間為 10 分鐘

Review: NCBI/Blast I, Blast II/Comparative Genetics, and Multiple Sequence Alignments

...
1 個測驗
1 個練習
Quiz: Modules 1-310分鐘
5
完成時間為 2 小時

Phylogenetics

In this module we'll be using the multiple sequence alignments we generated last lab to do some phylogenetic analyses with both neighbour-joining and maximum likelihood methods. The tree-like structure generated by such analyses tells us how closely sequences are related one to another, and suggests when in evolutionary time a speciation or gene duplication event occurred....
4 個視頻 (總計 36 分鐘), 2 個閱讀材料, 1 個測驗
4 個視頻
Lecture25分鐘
Lab Discussion9分鐘
Summary
2 個閱讀材料
Lecture Materials10分鐘
Lab 4 -- Phylogenetics30分鐘
1 個練習
Lab 4 Quiz6分鐘
6
完成時間為 2 小時

Selection Analysis

In this module we'll take a set of orthologous sequences from bacteria and use DataMonkey to analyze them for the presence of certain sites under positive, negative or neutral selection. Such an analysis can help understand the biology of a set of protein coding sequences by identifying residues that might be important for biological function (those residues under negative selection) or those that might be involved in response to external influences, such as drugs, pathogens or other factors (residues under positive selection)....
4 個視頻 (總計 38 分鐘), 2 個閱讀材料, 1 個測驗
4 個視頻
Lecture23分鐘
Lab Discussion10分鐘
Summary1分鐘
2 個閱讀材料
Lecture Materials10分鐘
Lab 5 -- Selection Analysis30分鐘
1 個練習
Lab 5 Quiz6分鐘
7
完成時間為 3 小時

'Next Gen' Sequence Analysis (RNA-Seq) / Metagenomics

In this module we'll explore some of the data that have been generated as a result of the rapid decrease in the cost of sequencing DNA. We'll be exploring a couple of RNA-Seq data sets that can tell us where any given gene is expressed, and also how that gene might be alternatively spliced. We'll also be looking at a couple of metagenome data sets that can tell us about the kinds of species (especially microbial species that might otherwise be hard to culture) that are in a given environmental niche....
4 個視頻 (總計 51 分鐘), 2 個閱讀材料, 1 個測驗
4 個視頻
Lecture24分鐘
Lab Discussion20分鐘
Summary4分鐘
2 個閱讀材料
Lecture Materials10分鐘
Lab 6 -- Next Generation Sequencing Applications: RNA-Seq and Metagenomics30分鐘
1 個練習
Lab 6 Quiz6分鐘
8
完成時間為 1 小時

Review: Phylogenetics, Selection Analysis, and 'Next Gen' Sequence Analysis (RNA-seq)/Metagenomics + Final Assignment

...
1 個閱讀材料, 2 個測驗
1 個閱讀材料
Final Assignment Instructions10分鐘
2 個練習
Review: Modules 5-710分鐘
Final Assignment10分鐘
4.7
158 個審閱Chevron Right

21%

完成這些課程後已開始新的職業生涯

29%

通過此課程獲得實實在在的工作福利

20%

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熱門審閱

創建者 EDApr 27th 2017

Great course. All lectures provide a biological context for the tools you learn in the labs. The labs themselves provide a great introduction to the many tools available for bioinformatic analysis.

創建者 RGNov 5th 2017

I enjoyed doing the course. It is exciting to see how much one can learn from a few gene or protein sequences. Thank you for making the course understandable to a beginner in bioinformatics!

講師

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Nicholas James Provart

Professor
Cell & Systems Biology

關於 多伦多大学

Established in 1827, the University of Toronto is one of the world’s leading universities, renowned for its excellence in teaching, research, innovation and entrepreneurship, as well as its impact on economic prosperity and social well-being around the globe. ...

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