課程信息
4.7
619 ratings
140 reviews
Large-scale biology projects such as the sequencing of the human genome and gene expression surveys using RNA-seq, microarrays and other technologies have created a wealth of data for biologists. However, the challenge facing scientists is analyzing and even accessing these data to extract useful information pertaining to the system being studied. This course focuses on employing existing bioinformatic resources – mainly web-based programs and databases – to access the wealth of data to answer questions relevant to the average biologist, and is highly hands-on. Topics covered include multiple sequence alignments, phylogenetics, gene expression data analysis, and protein interaction networks, in two separate parts. The first part, Bioinformatic Methods I (this one), deals with databases, Blast, multiple sequence alignments, phylogenetics, selection analysis and metagenomics. This, the second part, Bioinformatic Methods II, covers motif searching, protein-protein interactions, structural bioinformatics, gene expression data analysis, and cis-element predictions. This pair of courses is useful to any student considering graduate school in the biological sciences, as well as students considering molecular medicine. Both provide an overview of the many different bioinformatic tools that are out there. These courses are based on one taught at the University of Toronto to upper-level undergraduates who have some understanding of basic molecular biology. If you're not familiar with this, something like https://learn.saylor.org/course/bio101 might be helpful. No programming is required for this course....
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建議:12-18 hours videos and labs

完成時間大約為12 小時
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您將獲得的技能

Genetic AnalysisBioinformatics AnalysisEvolutionComparative Genomics
Globe

100% 在線課程

立即開始,按照自己的計劃學習。
Calendar

可靈活調整截止日期

根據您的日程表重置截止日期。
Clock

建議:12-18 hours videos and labs

完成時間大約為12 小時
Comment Dots

English

字幕:English

教學大綱 - 您將從這門課程中學到什麼

1

章節
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完成時間為 3 小時

NCBI/Blast I

In this module we'll be exploring the amazing resources available at NCBI, the National Centre for Biotechnology Information, run by the National Library of Medicine in the USA. We'll also be doing a Blast search to find similar sequences in the enormous NR sequence database. We can use similar sequences to infer homology, which is the primary predictor of gene or protein function. ...
Reading
4 個視頻(共 52 分鐘), 4 個閱讀材料, 1 個測驗
Video4 個視頻
Lecture25分鐘
Lab Discussion24分鐘
Summary分鐘
Reading4 個閱讀材料
Acknowledgements10分鐘
Course Logistics10分鐘
Lecture Materials10分鐘
Lab 1 -- Exploring NCBI30分鐘
Quiz1 個練習
Lab 1 Quiz6分鐘

2

章節
Clock
完成時間為 3 小時

Blast II/Comparative Genomics

In this module we'll continue exploring the incredible resources available at NCBI, the National Centre for Biotechnology Information. We will be performing several different kinds of Blast searches: BlastP, PSI-Blast, and Translated Blast. We can use similar sequences identified by such methods to infer homology, which is the primary predictor of gene or protein function. We'll also be comparing parts of the genomes of a couple of different species, to see how similar they are....
Reading
4 個視頻(共 58 分鐘), 2 個閱讀材料, 1 個測驗
Video4 個視頻
Lecture32分鐘
Lab Discussion23分鐘
Summary分鐘
Reading2 個閱讀材料
Lecture Materials10分鐘
Lab 2 -- Advanced Blast and Comparative Genomics30分鐘
Quiz1 個練習
Lab 2 Quiz6分鐘

3

章節
Clock
完成時間為 2 小時

Multiple Sequence Alignments

In this module we'll be doing multiple sequence alignments with Clustal (as implemented in MEGA), DiAlign, and MAFFT. Multiple sequences alignments can tell you where in a sequence the conserved and variable regions are, which is important for understanding the biology of the sequences under investigation. It also has practical applications, such as being able to design PCR primers that will amplify sequences from a number of different species, for example....
Reading
4 個視頻(共 42 分鐘), 2 個閱讀材料, 1 個測驗
Video4 個視頻
Lecture23分鐘
Lab Discussion16分鐘
Summary分鐘
Reading2 個閱讀材料
Lecture Materials10分鐘
Lab 3 -- Multiple Sequence Alignment30分鐘
Quiz1 個練習
Lab 3 Quiz6分鐘

4

章節
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完成時間為 10 分鐘

Review: NCBI/Blast I, Blast II/Comparative Genetics, and Multiple Sequence Alignments

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Reading
1 個測驗
Quiz1 個練習
Quiz: Modules 1-310分鐘
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創建者 EDApr 27th 2017

Great course. All lectures provide a biological context for the tools you learn in the labs. The labs themselves provide a great introduction to the many tools available for bioinformatic analysis.

創建者 FJFeb 6th 2017

The videos were very clear on how to use various bioinformatic tools. I found no difficulty in understanding them. Really enjoyed the course. Looking forward to the next part of this course!

講師

Nicholas James Provart

Professor
Cell & Systems Biology

關於 University of Toronto

Established in 1827, the University of Toronto has one of the strongest research and teaching faculties in North America, presenting top students at all levels with an intellectual environment unmatched in depth and breadth on any other Canadian campus. ...

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  • Once you enroll for a Certificate, you’ll have access to all videos, quizzes, and programming assignments (if applicable). Peer review assignments can only be submitted and reviewed once your session has begun. If you choose to explore the course without purchasing, you may not be able to access certain assignments.

  • When you purchase a Certificate you get access to all course materials, including graded assignments. Upon completing the course, your electronic Certificate will be added to your Accomplishments page - from there, you can print your Certificate or add it to your LinkedIn profile. If you only want to read and view the course content, you can audit the course for free.

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