Large-scale biology projects such as the sequencing of the human genome and gene expression surveys using RNA-seq, microarrays and other technologies have created a wealth of data for biologists. However, the challenge facing scientists is analyzing and even accessing these data to extract useful information pertaining to the system being studied. This course focuses on employing existing bioinformatic resources – mainly web-based programs and databases – to access the wealth of data to answer questions relevant to the average biologist, and is highly hands-on.
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課程信息
39,606 次近期查看
學生職業成果
24%
完成這些課程後已開始新的職業生涯
30%
通過此課程獲得實實在在的工作福利
17%
加薪或升職
您將獲得的技能
Genetic AnalysisBioinformatics AnalysisEvolutionComparative Genomics
學生職業成果
24%
完成這些課程後已開始新的職業生涯
30%
通過此課程獲得實實在在的工作福利
17%
加薪或升職
可分享的證書
完成後獲得證書
100% 在線
立即開始，按照自己的計劃學習。
第 1 門課程（共 4 門）
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初級
完成時間大約為13 小時
建議：12-18 hours videos and labs
英語（English）
字幕：英語（English）
教學大綱 - 您將從這門課程中學到什麼
完成時間為 3 小時
NCBI/Blast I
In this module we'll be exploring the amazing resources available at NCBI, the National Centre for Biotechnology Information, run by the National Library of Medicine in the USA. We'll also be doing a Blast search to find similar sequences in the enormous NR sequence database. We can use similar sequences to infer homology, which is the primary predictor of gene or protein function.
完成時間為 3 小時
4 個視頻 （總計 51 分鐘）, 4 個閱讀材料, 1 個測驗
4 個閱讀材料
Acknowledgements10分鐘
Course Logistics10分鐘
Lecture Materials10分鐘
Lab 1 -- Exploring NCBI1 小時 30 分
1 個練習
Lab 1 Quiz30分鐘
完成時間為 3 小時
Blast II/Comparative Genomics
In this module we'll continue exploring the incredible resources available at NCBI, the National Centre for Biotechnology Information. We will be performing several different kinds of Blast searches: BlastP, PSI-Blast, and Translated Blast. We can use similar sequences identified by such methods to infer homology, which is the primary predictor of gene or protein function. We'll also be comparing parts of the genomes of a couple of different species, to see how similar they are.
完成時間為 3 小時
4 個視頻 （總計 58 分鐘）, 2 個閱讀材料, 1 個測驗
2 個閱讀材料
Lecture Materials10分鐘
Lab 2 -- Advanced Blast and Comparative Genomics1 小時 30 分
1 個練習
Lab 2 Quiz30分鐘
完成時間為 3 小時
Multiple Sequence Alignments
In this module we'll be doing multiple sequence alignments with Clustal (as implemented in MEGA), DiAlign, and MAFFT. Multiple sequences alignments can tell you where in a sequence the conserved and variable regions are, which is important for understanding the biology of the sequences under investigation. It also has practical applications, such as being able to design PCR primers that will amplify sequences from a number of different species, for example.
完成時間為 3 小時
4 個視頻 （總計 44 分鐘）, 2 個閱讀材料, 1 個測驗
2 個閱讀材料
Lecture Materials10分鐘
Lab 3 -- Multiple Sequence Alignment1 小時 30 分
1 個練習
Lab 3 Quiz30分鐘
完成時間為 1 小時
Review: NCBI/Blast I, Blast II/Comparative Genetics, and Multiple Sequence Alignments
完成時間為 1 小時
1 個練習
Quiz: Modules 1-330分鐘
審閱
4.7
來自生物信息学方法 I的熱門評論
由 ED 提供Apr 26, 2017
Great course. All lectures provide a biological context for the tools you learn in the labs. The labs themselves provide a great introduction to the many tools available for bioinformatic analysis.
由 FJ 提供Feb 5, 2017
The videos were very clear on how to use various bioinformatic tools. I found no difficulty in understanding them. Really enjoyed the course. Looking forward to the next part of this course!
由 RG 提供Nov 4, 2017
I enjoyed doing the course. It is exciting to see how much one can learn from a few gene or protein sequences. Thank you for making the course understandable to a beginner in bioinformatics!
由 PS 提供Jul 8, 2017
A fully recommended course for those who wish to understand the basics and strengthen their skills in Bioinformatics. A well-designed and precise course. Thank you Dr. Nicholas Provart. :-)
關於 多伦多大学
Established in 1827, the University of Toronto is one of the world’s leading universities, renowned for its excellence in teaching, research, innovation and entrepreneurship, as well as its impact on economic prosperity and social well-being around the globe.
關於 Plant Bioinformatic Methods 專項課程
The past 15 years have been exciting ones in plant biology. Hundreds of plant genomes have been sequenced, RNA-seq has enabled transcriptome-wide expression profiling, and a proliferation of "-seq"-based methods has permitted protein-protein and protein-DNA interactions to be determined cheaply and in a high-throughput manner. These data sets in turn allow us to generate hypotheses at the click of a mouse or tap of a finger.The Plant Bioinformatics Specialization on Coursera introduces core bioinformatic competencies and resources, such as NCBI's Genbank, Blast, multiple sequence alignments, phylogenetics in Bioinformatic Methods I, followed by protein-protein interaction, structural bioinformatics and RNA-seq analysis in Bioinformatic Methods II. In Plant Bioinformatics we cover 33 plant-specific online tools from genome browsers to transcriptomic data mining to promoter/network analyses and others. Last, a Plant Bioinformatics Capstone uses these tools to hypothesize a biological role for a gene of unknown function, summarized in a written lab report.This specialization is useful to any modern plant molecular biologist wanting to get a feeling for the incredible scope of data available to researchers. A small amount of R programming is introduced in Bioinformatic Methods II, but most of the tools are web applications. It is recommended that you have access to a laptop or desktop computer for running these as they may not work as mobile applications on your phone or tablet.
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