課程概述
The Library of Integrative Network-based Cellular Signatures (LINCS) is an NIH Common Fund program. The idea is to perturb different types of human cells with many different types of perturbations such as: drugs and other small molecules; genetic manipulations such as knockdown or overexpression of single genes; manipulation of the extracellular microenvironment conditions, for example, growing cells on different surfaces, and more. These perturbations are applied to various types of human cells including induced pluripotent stem cells from patients, differentiated into various lineages such as neurons or cardiomyocytes. Then, to better understand the molecular networks that are affected by these perturbations, changes in level of many different variables are measured including: mRNAs, proteins, and metabolites, as well as cellular phenotypic changes such as changes in cell morphology. The BD2K-LINCS Data Coordination and Integration Center (DCIC) is commissioned to organize, analyze, visualize and integrate this data with other publicly available relevant resources. In this course we briefly introduce the DCIC and the various Centers that collect data for LINCS. We then cover metadata and how metadata is linked to ontologies. We then present data processing and normalization methods to clean and harmonize LINCS data. This follow discussions about how data is served as RESTful APIs. Most importantly, the course covers computational methods including: data clustering, gene-set enrichment analysis, interactive data visualization, and supervised learning. Finally, we introduce crowdsourcing/citizen-science projects where students can work together in teams to extract expression signatures from public databases and then query such collections of signatures against LINCS data for predicting small molecules as potential therapeutics.
熱門審閱
MS
Jan 21, 2017
A very practical courses. Very good introduction to Big Data sources and Computational Analysis tool.
HH
Sep 19, 2018
excellent oppurtunity for the data science learners
篩選依據：
1 - 大数据科学与 BD2K-LINCS 数据协调和集成中心 的 4 個評論（共 4 個）
創建者 Musalula S
•Jan 21, 2017
A very practical courses. Very good introduction to Big Data sources and Computational Analysis tool.
創建者 Jose M V
•Jul 29, 2017
I recommend it, good materials, very instructive and a good level. Very interesting course.
創建者 Hemasree
•Sep 19, 2018
excellent oppurtunity for the data science learners
創建者 Ezra L
•Feb 28, 2018
Fun, fascinating, and informative!
